LSBio
Polyclonal Rabbit anti-Mouse PAX7 Antibody (Internal, WB)

Western Blot of rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder Lane 2: Mouse muscle protein lysate. Lane 3: C2C12. Lane 4: Ms spleen. Lane 5: Ms brain. Lane 6: HEK293T. Lane 7: HeLa WCL. Lane 8: MCF7. Lane 9: Jurkat. Lane 10: A431. Lane 11: A549. Lane 12: LNCap. Lane 13: MOLT-4. Lane 14: Ramos. Lane 15: Raji. Lane 16: Opal Pre-stained ladder Load: 10 µg per lane. Primary antibody: Pax7 antibody at 1:1,000 for overnight at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa for Pax7.

Polyclonal Rabbit anti-Mouse PAX7 Antibody (Internal, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Mouse
Format:Liquid
See all Mouse PAX7 antibodies →
$311.00each
Catalog Number: LS-C745394-25

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Overview

Description
PAX7 antibody LS-C745394 is an unconjugated rabbit polyclonal antibody to mouse PAX7 (Internal). Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
HUP1 | Paired box 7 | Paired box gene 7 | PAX7 | Paired box homeotic gene 7 | Paired box protein Pax-7 | RMS2 | Paired domain gene 7 | PAX7 transcriptional factor | PAX7B
UniProt Number
P23759
NCBI GenBank
NM_002584 | NP_002575.1
SwissProt
P23759
Recommended Dilution
ApplicationDilution
ELISA1:10000 - 1:40000
WB1 µg/ml

Target

Target
Mouse PAX7
Antigen Species
Mouse
Epitope
Cross-reactivity with Pax7 from other sources has not been determined.
Gene Name
PAX7

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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