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PAX7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

PAX7 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G114213-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PAX7 (NM_013945), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HUP1 | Paired box 7 | Paired box gene 7 | PAX7 | Paired box homeotic gene 7 | Paired box protein Pax-7 | RMS2 | Paired domain gene 7 | PAX7 transcriptional factor | PAX7B
Origin Species
Human
NCBI GenBank
NM_002584
SwissProt
P23759

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAALPGTVPR MMRPAPGQNY PRTGFPLEVS TPLGQGRVNQ LGGVFINGRP LPNHIRHKIV
61EMAHHGIRPC VISRQLRVSH GCVSKILCRY QETGSIRPGA IGGSKPRQVA TPDVEKKIEE
121YKRENPGMFS WEIRDRLLKD GHCDRSTVPS VSSISRVLRI KFGKKEEEDE ADKKEDDGEK
181KAKHSIDGIL GDKGNRLDEG SDVESEPDLP LKRKQRRSRT TFTAEQLEEL EKAFERTHYP
241DIYTREELAQ RTKLTEARVQ VWFSNRRARW RKQAGANQLA AFNHLLPGGF PPTGMPTLPP
301YQLPDSTYPT TTISQDGGST VHRPQPLPPS TMHQGGLAAA AAAADTSSAY GARHSFSSYS
361DSFMNPAAPS NHMNPVSNGL SPQVMSILGN PSAVPPQPQA DFSISPLHGG LDSATSISAS
421CSQRADSIKP GDSLPTSQAY CPPTYSTTGY SVDPVAGYQY GQYGQSECLV PWASPVPIPS
481PTPRASCLFM ESYKVVSGWG MSISQMEKLK SSQMEQFT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

518 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.