Western Blot of rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder Lane 2: Mouse muscle protein lysate. Lane 3: C2C12. Lane 4: Ms spleen. Lane 5: Ms brain. Lane 6: HEK293T. Lane 7: HeLa WCL. Lane 8: MCF7. Lane 9: Jurkat. Lane 10: A431. Lane 11: A549. Lane 12: LNCap. Lane 13: MOLT-4. Lane 14: Ramos. Lane 15: Raji. Lane 16: Opal Pre-stained ladder Load: 10 µg per lane. Primary antibody: Pax7 antibody at 1:1,000 for overnight at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa for Pax7.

PAX7 Antibody (Internal)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:	Mouse
Format:	Liquid

$579.00each

Catalog Number: LS-C745393-100

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Overview

Description: PAX7 antibody LS-C745393 is an unconjugated rabbit polyclonal antibody to mouse PAX7 (Internal). Validated for ELISA and WB.
Application: ELISA, WB
Reactivity: Ms
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: HUP1 | Paired box 7 | Paired box gene 7 | PAX7 | Paired box homeotic gene 7 | Paired box protein Pax-7 | RMS2 | Paired domain gene 7 | PAX7 transcriptional factor | PAX7B
Recommended Dilution: ELISA: 1:10000 - 1:40000
Recommended Dilution: WB: 1 µg/ml
UniProt Number: P23759
NCBI GenBank: NM_002584 | NP_002575.1
SwissProt: P23759

Target

Target: Mouse PAX7
Antigen Species: Mouse
Epitope: Cross-reactivity with Pax7 from other sources has not been determined.
Gene Name: PAX7

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Isotype: IgG
Origin Species: Human

Immunogen

Immunogen: Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Affinity Purified

Storage & Handling

Storage Conditions: Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate|0.15 M NaCl