Human Apolipoprotein H Antibody (IgA) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67373-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder Lane 2: Mouse muscle protein lysate. Lane 3: C2C12. Lane 4: Ms spleen. Lane 5: Ms brain. Lane 6: HEK293T. Lane 7: HeLa WCL. Lane 8: MCF7. Lane 9: Jurkat. Lane 10: A431. Lane 11: A549. Lane 12: LNCap. Lane 13: MOLT-4. Lane 14: Ramos. Lane 15: Raji. Lane 16: Opal Pre-stained ladder Load: 10 µg per lane. Primary antibody: Pax7 antibody at 1:1,000 for overnight at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:70,000 for 30 min at RT. Blocking Buffer: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa for Pax7.
PAX7 Antibody (Internal)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Mouse |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C745393-100
Catalog Number: LS-C745393
For bulk orders or custom formulations:
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- Description
- PAX7 antibody LS-C745393 is an unconjugated rabbit polyclonal antibody to mouse PAX7 (Internal). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- HUP1 | Paired box 7 | Paired box gene 7 | PAX7 | Paired box homeotic gene 7 | Paired box protein Pax-7 | RMS2 | Paired domain gene 7 | PAX7 transcriptional factor | PAX7B
- UniProt Number
- P23759
- NCBI GenBank
- NM_002584 | NP_002575.1
- SwissProt
- P23759
Recommended Dilution
| Application | Dilution |
|---|---|
| ELISA | 1:10000 - 1:40000 |
| WB | 1 µg/ml |
Target
- Target
- Mouse PAX7
- Antigen Species
- Mouse
- Epitope
- Cross-reactivity with Pax7 from other sources has not been determined.
- Gene Name
- PAX7
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
