LSBio
Polyclonal Rabbit anti-Human TNF Alpha Antibody (IHC, WB)

TNF-alpha staining in human mammary cancer. Paraffin-embedded human mammary cancer is stained with TNF-alpha used at 1:200 dilution.

Polyclonal Rabbit anti-Human TNF Alpha Antibody (IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human TNF Alpha antibodies →
$441.00each
Catalog Number: LS-C415305-0.1

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Overview

Description
TNF Alpha antibody LS-C415305 is an unconjugated rabbit polyclonal antibody to TNF Alpha from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Application
ELISA, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: ELISA
1:1000 - 1:5000
Recommended Dilution: IHC
1:1000 - 1:2000
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P01375
SwissProt
P01375

Target

Target
Human TNF Alpha
Antigen Species
Human
Epitope
Tumor necrosis factor alpha (TNF-alpha) is a cytokine that binds to the TNF-R1 and TNF-R2 receptors. TNF-alpha is mainly secreted by macrophages and induces cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of IL-1 secretion, and therefore found in cachexia accompanying diverse cancers and infections. Genetic variations in TNF are associated with susceptibility to psoriatic arthritis and to HBV infection.
Gene Name
TNF

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
KLH-conjugated synthetic peptide from the C-Terminus of human TNF-alpha.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 2% BSA, 0.09% sodium azide.
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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