LSBio
IHC-plus™ TNF Alpha Antibody (clone M 1 C4)

Anti-TNF Alpha antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ TNF Alpha Antibody (clone M 1 C4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Rat
Format:Liquid
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$383.00each
Catalog Number: LS-B6067-50

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Overview

Description
TNF Alpha antibody LS-B6067 is an unconjugated mouse monoclonal antibody to TNF Alpha from human. It is reactive with human and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P01375
NCBI GenBank
NM_000594 | NP_000585.2
SwissProt
P01375

Target

Target
TNF Alpha
Antigen Species
Human
Specificity
Human TNF
Epitope
Human TNF
Gene Name
TNF

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
M 1 C4
Light Chain
Kappa Light Chain
Origin Species
Human

Immunogen

Immunogen
TNF (AAH28148, 1 aa ~ 234 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4
Concentration
0.37 mg/mL
Animal Free
No
Carrier-Free
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Inhibition of lncRNA H19/miR-370-3p pathway mitigates neuronal apoptosis in an in vitro model of spinal cord injury (SCI). Xin Li, Yan Qian, Kaihua Tang, Yang Li, Rui Tao, Chunyan Gong, Li Huang, Kaiwen Zou, Lindong Liu. Translational neuroscience. 2021 Mar;12:103-113. PubMed: 33708438 PMC: PMC7925972
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