Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Human TNFa Antibody - Immunohistochemistry. Fluorescent immunohistochemistry showing staining of human colon by anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor 680 goat polyclonal secondary antibody was used diluted 1:5000.
Polyclonal Rabbit anti-Human TNF Alpha Antibody (IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Radioimmunoassay, Western Blot |
| Reactivity: | Human, Non-Human Primate |
| Format: | Liquid |
$1250.00each
Catalog Number: LS-C59380-1
Catalog Number: LS-C59380
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- TNF Alpha antibody LS-C59380 is an unconjugated rabbit polyclonal antibody to TNF Alpha from human. It is reactive with human and primate. Validated for ELISA, IF, IHC and WB.
- Application
- ELISA, IF, IHC, IP, RIA, WB
- Reactivity
- Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- APC1 protein | Cachectin | DIF | TNF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
- Recommended Dilution: ELISA
- 1:1000 - 1:5000
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: IHC
- 1:100 - 1:500
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- P01375
- NCBI GenBank
- NM_000594 | NP_000585.2
- SwissProt
- P01375
Target
- Target
- Human TNF Alpha
- Antigen Species
- Human
- Epitope
- The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFb (lymphotoxin). This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
- Gene Name
- TNF
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum
- Concentration
- 75 mg/mL
- Volume
- 1 Milliliter
- Preservative
- No
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Short term: -20°C; Long term: -20°C.
