SMAD4 Antibody (N-Terminus)
Catalog Number: LS-B11491-100
100 µL (0.5 mg/mL) / $383.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~60.4 kDa SMAD4 protein using Rabbit Anti-SMAD4 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SMAD4 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~60.4 kDa.
Polyclonal Rabbit anti-Human SMAD4 Antibody (Biotin, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$485.00each
Catalog Number: LS-C774950-100
Catalog Number: LS-C774950
For bulk orders or custom formulations:
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- Description
- SMAD4 antibody LS-C774950 is a biotin-conjugated rabbit polyclonal antibody to human SMAD4. Validated for IHC and WB.
- Application
- IHC, WB
- Reactivity
- Hu
- Intended Use
- RUO
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DPC4 | HSMAD4 | MYHRS | SMAD 4 | MAD homolog 4 | SMAD family member 4 | SMAD4 | JIP | MADH4 | Mothers against DPP homolog 4
- Recommended Dilution: IHC
- 1:50
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- Q13485
- NCBI GenBank
- NM_005359, NP_005350.1
- SwissProt
- Q13485
Target
- Target
- Human SMAD4
- Antigen Species
- Hu
- Epitope
- Detects 60 kDa.
- Gene Name
- SMAD4
Antibody
- Host Species
- Rb
- Clonality
- Polyclonal
- Origin Species
- Hu
Immunogen
- Immunogen
- Synthetic peptide of Human SMAD4 (200-300 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
- Immunogen Type
- SP-Lin
Format
- Conjugate/Label
- Biotin
- Format
- Liquid
- Formulation
- PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- AP
Storage & Handling
- Storage Conditions
- Store at -20°C.
- Recommended Storage Buffer
- PBS
