Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
ELISA results of purified Rabbit anti-PPAR gamma pS273 tested against BSA-conjugated non-phospho and phospho forms of immunizing peptide. Each well was coated in duplicate with either 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB substrate
Polyclonal Rabbit anti-Human PPARG / PPAR Gamma Antibody (phospho-Ser273, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$579.00each
Catalog Number: LS-C209422-50
Catalog Number: LS-C209422
For bulk orders or custom formulations:
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- Description
- PPAR Gamma antibody LS-C209422 is an unconjugated rabbit polyclonal antibody to human PPAR Gamma (PPARG) (pSer273). Validated for ELISA, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Dog, Mouse, Non-Human Primate, Pig, Rat
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- NR1C3 | PPAR gamma | PPARG | PPAR gamma 2 | PPARG1 | PPARG2 | PPARgamma | CIMT1 | GLM1 | PPAR-gamma
- Recommended Dilution: ELISA
- 1:10000
- UniProt Number
- P37231
- NCBI GenBank
- NM_015869 | NP_056953.2
- SwissProt
- P37231
Target
- Target
- Human PPARG / PPAR Gamma
- Antigen Species
- Human
- Epitope
- A BLAST analysis was used to suggest cross-reactivity with rat, mouse, primate, human, dog, bovine, and pig based on 100% sequence homology. Cross-reactivity with PPAR gamma pS273 from other sources has not been determined.
- Gene Name
- PPARG
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Anti-PPAR gamma pS273 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the internal region of human PPARG protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.05% Sodium Azide
- Concentration
- 0.85 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
PPAR? Interaction with UBR5/ATMIN Promotes DNA Repair to Maintain Endothelial Homeostasis. Li CG, Mahon C, Sweeney NM, Verschueren E, Kantamani V, Li D, Hennigs JK, Marciano DP, Diebold I, Abu-Halawa O, Elliott M, Sa S, Guo F, Wang L, Cao A, Guignabert C, Sollier J, Nickel NP, Kaschwich M, Cimprich KA, Rabinovitch M. Cell reports. 2019 January;26:1333-1343. PubMed: 30699358 PMC: PMC6436616
