LSBio
IHC-plus™ PPARG / PPAR Gamma Antibody

Anti-PPARG antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ PPARG / PPAR Gamma Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Non-Human Primate
Format:Liquid
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$526.00each
Catalog Number: LS-B130-50

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Overview

Description
PPAR Gamma antibody LS-B130 is an unconjugated rabbit polyclonal antibody to PPAR Gamma (PPARG) from human. It is reactive with human and macaque. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu, NHP
Predicted Reactivity
Non-Human Primate, Pig
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
NR1C3 | PPAR gamma | PPARG | PPAR gamma 2 | PPARG1 | PPARG2 | PPARgamma | CIMT1 | GLM1 | PPAR-gamma
Recommended Dilution: ELISA
1:4000 - 1:40000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: WB
1:1000 - 1:3000
UniProt Number
P37231
SwissProt
P37231

Target

Target
PPARG / PPAR Gamma
Antigen Species
Human
Specificity
A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from mouse. Mouse PPARg2 shows 87% homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Epitope
A BLAST analysis was used to suggest reactivity with this protein from human and macaque based on 100% homology for the immunogen sequence. Cross reactivity with PPARg2 protein from dog and swine is likely due to 94% homology (15/16 identities) with the protein from these sources. Cross reactivity does occur with PPARg2 from mouse. Mouse PPARg2 shows 87% homology to the immunogen. Cross reactivity with PPARg2 homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
Gene Name
PPARG

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human PPAR gamma 2.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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