LSBio
IHC-plus™ PPARG / PPAR Gamma Antibody (clone 8D1H8F4)

Anti-PPARG antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ PPARG / PPAR Gamma Antibody (clone 8D1H8F4)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
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$470.00each
Catalog Number: LS-B7391-50

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Overview

Description
PPAR Gamma antibody LS-B7391 is an unconjugated mouse monoclonal antibody to human PPAR Gamma (PPARG). Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
NR1C3 | PPAR gamma | PPARG | PPAR gamma 2 | PPARG1 | PPARG2 | PPARgamma | CIMT1 | GLM1 | PPAR-gamma
Recommended Dilution: ELISA
1:10000
Recommended Dilution: IF/ICC
1:200 - 1:1000
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P37231
SwissProt
P37231

Target

Target
PPARG / PPAR Gamma
Antigen Species
Human
Specificity
Ni-NTA purified truncated recombinant PPARG expressed in E. Coli strain BL21 (DE3)
Epitope
Ni-NTA purified truncated recombinant PPARG expressed in E. Coli strain BL21 (DE3)
Gene Name
PPARG

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
8D1H8F4
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Recombinant human PPARG.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
Ascites fluid.
Concentration
0.5 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Purification Method
Unpurified (Serum / Ascites / Supernatant)

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
TAZ Is a Negative Regulator of PPAR? Activity in Adipocytes and TAZ Deletion Improves Insulin Sensitivity and Glucose Tolerance. Dalila El Ouarrat, Roi Isaac, Yun Sok Lee, Da Young Oh, Joshua Wollam, Denise Lackey, Matthew Riopel, Gautam Bandyopadhyay, Jong Bae Seo, Revathy Sampath-Kumar, Jerrold M Olefsky. Cell metabolism. 2020 January;31:162-173.e5. PubMed: 31708444 PMC: PMC7784082

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