LSBio
Polyclonal Rabbit anti-Human MITF Antibody (phospho-Ser180,phospho-Ser73, IHC, IF, WB)

1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

Polyclonal Rabbit anti-Human MITF Antibody (phospho-Ser180,phospho-Ser73, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Non-Human Primate, Rat
Format:Liquid
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$288.00each
Catalog Number: LS-C800889-100

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Overview

Description
MITF antibody LS-C800889 is an unconjugated rabbit polyclonal antibody to MITF (pSer180,pSer73) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, Peptide-ELISA and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, NHP, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BHLHe32 | CMM8 | MI | MITF | Waardenburg syndrome, type 2A | WS2 | WS2A
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
O75030
NCBI GenBank
NM_000248 | NP_000239.1
SwissProt
O75030

Target

Target
Human MITF
Antigen Species
Human
Epitope
Phospho-MITF (Ser180/73) Antibody detects endogenous levels of MITF only when phosphorylated at Serine 180/73.
Gene Name
MITF

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthesized peptide derived from human MITF around the phosphorylation site of Serine 180/73.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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