LSBio
Polyclonal Rabbit anti-Human MITF Antibody (IHC, IF, WB)

Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

Polyclonal Rabbit anti-Human MITF Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Non-Human Primate
Format:Liquid
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$288.00each
Catalog Number: LS-C808611-100

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Overview

Description
MITF antibody LS-C808611 is an unconjugated rabbit polyclonal antibody to MITF from human. It is reactive with human, mouse and monkey. Validated for IF, IHC, Peptide-ELISA and WB.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, NHP
Intended Use
RUO
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
BHLHe32 | CMM8 | MI | MITF | Waardenburg syndrome, type 2A | WS2 | WS2A
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
O75030
SwissProt
O75030

Target

Target
Human MITF
Antigen Species
Hu
Epitope
MITF Antibody detects endogenous levels of total MITF.
Gene Name
MITF

Antibody

Host Species
Rb
Clonality
Polyclonal
Isotype
IgG
Origin Species
Hu

Immunogen

Immunogen
A synthesized peptide derived from human MITF.
Immunogen Type
SP-Lin

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
AP

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

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