Polyclonal Rabbit anti-Human SLC5A11 / SMIT2 Antibody (WB)
Catalog Number: LS-C748223
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Polyclonal Rabbit anti-Human IL-1B / IL-1 Beta Antibody (N-Terminus, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Neutralization, Radioimmunoassay, Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate |
| Format: | Liquid |
$1250.00each
Catalog Number: LS-C18827-1
Catalog Number: LS-C18827
For bulk orders or custom formulations:
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- Description
- IL-1 Beta antibody LS-C18827 is an unconjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) (N-Terminus) from human. It is reactive with human, dog and primate. Validated for ELISA, Flow, IF, IHC, IP, Neut, RIA and WB. Cited in 2 publications.
- Application
- ELISA, FC, IF, IHC, IHC-F, IHC-P, IP, Neut, RIA, WB
- Reactivity
- Can, Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- IL-1B | IL1B | IL-1 beta | IL1-BETA | Preinterleukin 1 beta | Pro-interleukin-1-beta | Catabolin | IL-1 | IL1F2 | Interleukin 1, beta | Interleukin-1 beta
- Recommended Dilution: ELISA
- 1:500 - 1:2000
- Recommended Dilution: IHC-F
- 1:100 - 1:200
- Recommended Dilution: IHC-P
- 1:100 - 1:200
- Recommended Dilution: IP
- 1:400 - 1:800
- Recommended Dilution: WB
- 1:1000
- UniProt Number
- P01584
- NCBI GenBank
- NM_000576 | NP_000567.1
- SwissProt
- P01584
Target
- Target
- Human IL-1B / IL-1 Beta
- Antigen Species
- Human
- Epitope
- This antibody is primarily directed against mature, 17,000 MW human IL-1β and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1β in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1β containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1β precursor molecule. Denatured 31,000 precursor IL-1β will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1β exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1β precursor in lysates.
- Gene Name
- IL1B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was prepared by repeated immunizations with recombinant human IL-1β produced in E.coli. The MW of the recombinant 153 aa IL-1β was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1β converting enzyme (ICE, capase-1).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
- Concentration
- 2 mg/mL
- Preservative
- No
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Serum soluble interleukin 7 receptor is strongly associated with lupus nephritis in patients with systemic lupus erythematosus. Badot V, Luijten RK, van Roon JA, Depresseux G, Aydin S, Van den Eynde BJ, Houssiau FA, Lauwerys BR. Annals of the rheumatic diseases. 2013;72:453-6.
PubMed: 23264357
p65-Dependent production of interleukin-1 beta by osteolytic prostate cancer cells causes an induction of chemokine expression in osteoblasts. Schulze J, Weber K, Baranowsky A, Streichert T, Lange T, Spiro AS, Albers J, Seitz S, Zustin J, Amling M, Fehse B, Schinke T. Cancer letters. 2012 Apr;317:106-13.
PubMed: 22108531
