LSBio
Monoclonal Mouse anti-Human IL-1B / IL-1 Beta Antibody (clone OTI3E1, IHC, IF, WB)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL1B.

Monoclonal Mouse anti-Human IL-1B / IL-1 Beta Antibody (clone OTI3E1, IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all Human IL-1B / IL-1 Beta antibodies →
$459.00each
Catalog Number: LS-C174781-100

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Overview

Description
IL-1 Beta antibody LS-C174781 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for IF, IHC and WB. Cited in 1 publication.
Application
IF, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
IL-1B | IL1B | IL-1 beta | IL1-BETA | Preinterleukin 1 beta | Pro-interleukin-1-beta | Catabolin | IL-1 | IL1F2 | Interleukin 1, beta | Interleukin-1 beta
Recommended Dilution: IF/ICC
1:100
Recommended Dilution: IHC-P
1:150
Recommended Dilution: WB
1:1000 - 1:4000
UniProt Number
P01584
SwissProt
P01584

Target

Target
Human IL-1B / IL-1 Beta
Antigen Species
Human
Epitope
Human IL1B / IL-1 Beta
Gene Name
IL1B

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OTI3E1
Isotype
IgG1

Immunogen

Immunogen
Full length human recombinant protein of human IL1B(NP_000567) produced in HEK293T cell.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Cold-induced urticarial autoinflammatory syndrome related to factor XII activation. Jörg Scheffel, Niklas A Mahnke, Zonne L M Hofman, Steven de Maat, Jim Wu, Hanna Bonnekoh, Reuben J Pengelly, Sarah Ennis, John W Holloway, Marieluise Kirchner, Philipp Mertins, Martin K Church, Marcus Maurer, Coen Maas, Karoline Krause. Nature communications. 2020 Jan;11:179. PubMed: 31924766 PMC: PMC6954242

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