This antibody is primarily directed against mature, 17,000 MW human IL-1 and is useful in determining its presence in various assays.
IL-1B / IL-1 Beta Antibody (N-Terminus, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Immunohistochemistry (general), Immunoprecipitation, Neutralization, Radioimmunoassay, Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate |
| Format: | Lyophilized |
$564.00each
Catalog Number: LS-C154106-100
Catalog Number: LS-C154106
For bulk orders or custom formulations:
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- Description
- IL-1 Beta antibody LS-C154106 is an HRP-conjugated rabbit polyclonal antibody to IL-1 Beta (IL-1B) (N-Terminus) from human. It is reactive with human, dog and primate. Validated for ELISA, Flow, IF, IHC, IP, Neut, RIA and WB.
- Application
- ELISA, FC, IF, IHC, IP, Neut, RIA, WB
- Reactivity
- Can, Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- IL-1B | IL1B | IL-1 beta | IL1-BETA | Preinterleukin 1 beta | Pro-interleukin-1-beta | Catabolin | IL-1 | IL1F2 | Interleukin 1, beta | Interleukin-1 beta
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IHC
- 1:500 - 1:2500
- Recommended Dilution: WB
- 1:1000 - 1:5000
- UniProt Number
- P01584
- NCBI GenBank
- NM_000576 | NP_000567.1
- SwissProt
- P01584
Target
- Target
- Human IL-1B / IL-1 Beta
- Antigen Species
- Human
- Epitope
- This antibody was heated to 56° C for 30 minutes. This is an IgG preparation of whole rabbit serum purified by DEAE fractionation. This antibody is primarily directed against mature, 17,000 MW human IL-1β and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1β in the same formats using similar dilutions. The antiserum does not recognize human IL-1a. In ELISA formats and other immunoreactive assays, this antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW IL-1β containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis of natural cell products or human body fluids, the usual procedure of hearing the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1β precursor molecule. Denatured 31,000 precursor IL-1β will be recognized by this antibody but often migrates as a 35,000 MW band. This is due to the unfolding of the denatured precursor IL-1β exposing epitopes not exposed in the natural state. In immunoblots, depending on the number of cells, the antibody detects the 17,000 MW band in supernatants as well as a 35,000 MW band representing the 31,000 MW IL-1β precursor in lysates.
- Gene Name
- IL1B
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- This antibody was prepared by repeated immunizations with recombinant human IL-1β produced in E.coli. The MW of the recombinant 153 aa IL-1β was 17 kDa with the N-terminal amino acid at position alanine 117. This cleavage site is generated by the IL-1β converting enzyme (ICE, capase-1).
- Immunogen Type
- Recombinant Immunogen
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1.1 mg/mL
- Preservative
- Yes
- Purification Method
- Ion Exchange Chromatography
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl