LSBio
Polyclonal Rabbit anti-Human HAUS8 Antibody (WB)

Western Blot of rabbit Anti-Hice1 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 µg per lane. Primary antibody: Hice1 antibody at 0.5 µg/mL for overnight at 4°C. Secondary antibody: Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1. Other band(s): none.

Polyclonal Rabbit anti-Human HAUS8 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity:Human
Format:Liquid
See all Human HAUS8 antibodies →
$311.00each
Catalog Number: LS-C745349-25

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Overview

Description
HAUS8 antibody LS-C745349 is an unconjugated rabbit polyclonal antibody to human HAUS8. Validated for ELISA and WB.
Application
ELISA, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DGT4 | HAUS8 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
Recommended Dilution: ELISA
1:30000 - 1:45000
Recommended Dilution: WB
0.5 µg/ml
UniProt Number
Q9BT25
SwissProt
Q9BT25

Target

Target
Human HAUS8
Antigen Species
Human
Epitope
A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Gene Name
HAUS8

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
Anti-Hice1 Antibody was prepared by repeated immunizations with a synthetic peptide corresponding to the region of amino acids containing serine 70 of human Hice1.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1.06 mg/mL
Volume
25 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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