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Polyclonal Rabbit anti-Human HAUS8 Antibody (WB)

Anti-HICE1 in Western blot of Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

Polyclonal Rabbit anti-Human HAUS8 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:Human
Format:Liquid
See all Human HAUS8 antibodies →
$579.00each
Catalog Number: LS-C153765-100

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Overview

Description
HAUS8 antibody LS-C153765 is an unconjugated rabbit polyclonal antibody to human HAUS8. Validated for ELISA and WB.
Application
ELISA, IHC, WB
Reactivity
Hu
Predicted Reactivity
Non-Human Primate
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
DGT4 | HAUS8 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
Recommended Dilution: ELISA
1:250000
Recommended Dilution: WB
1:10000
UniProt Number
Q9BT25
SwissProt
Q9BT25

Target

Target
Human HAUS8
Antigen Species
Human
Epitope
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.
Gene Name
HAUS8

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
38 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

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