Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western Blot of rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 µg per lane. Primary antibody: Hice1 pS70 antibody at 0.5 µg/mL for overnight at 4°C. Secondary antibody: Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band(s): none.
HAUS8 Antibody (phospho-Ser70)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$311.00each
Catalog Number: LS-C745347-25
Catalog Number: LS-C745347
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HAUS8 antibody LS-C745347 is an unconjugated rabbit polyclonal antibody to human HAUS8 (pSer70). Validated for ELISA and WB.
- Application
- ELISA, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- DGT4 | HAUS8 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
- Recommended Dilution: ELISA
- 1:17000 - 1:45000
- Recommended Dilution: WB
- 0.5 µg/ml
- UniProt Number
- Q9BT25
- SwissProt
- Q9BT25
Target
- Target
- Human HAUS8
- Antigen Species
- Human
- Epitope
- The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
- Gene Name
- HAUS8
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 0.47 mg/mL
- Volume
- 25 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
