LSBio
Polyclonal Rabbit anti-Human COL2A1 / Collagen II Alpha 1 Antibody (IHC, IF, WB)

1:200 staining human liver cells by IF/ICC. cells were formaldehyde fixed, permeabilized by Triton X-100 and blocked 5% BSA for 30 min at room temperature. The sample was incubated with the primary antibody (1:200 in BSA) for 1 hour. An Alexa Fluor 488®-conjugated Goat anti-rabbit antibody was used as the secondary.

Polyclonal Rabbit anti-Human COL2A1 / Collagen II Alpha 1 Antibody (IHC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all Human COL2A1 / Collagen II Alpha 1 antibodies →
$288.00each
Catalog Number: LS-C800256-100

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Overview

Description
Collagen II Alpha 1 antibody LS-C800256 is an unconjugated rabbit polyclonal antibody to Collagen II Alpha 1 (COL2A1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Cited in 1 publication.
Application
ELISA, IF, IHC, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Alpha-1 type II collagen | Cartilage collagen | COL11A3 | Collagen alpha 1(ii) | Collagen, type II, alpha 1 | Collagen alpha-1(II) chain | Chondrocalcin | ANFH | AOM | COL2A1 | SEDC
Recommended Dilution: IHC
1:50 - 1:200
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
P02458
SwissProt
P02458

Target

Target
Human COL2A1 / Collagen II Alpha 1
Antigen Species
Human
Epitope
Collagen II antibody detects endogenous levels of total Collagen II.
Gene Name
COL2A1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Origin Species
Human

Immunogen

Immunogen
A synthesized peptide derived from human Collagen II.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Concentration
1 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
MiR-99a alleviates apoptosis and extracellular matrix degradation in experimentally induced spine osteoarthritis by targeting FZD8. Yeyang Wang, Xiaoyu Zheng, Dixin Luo, Wangyang Xu, Xiaozhong Zhou. BMC musculoskeletal disorders. 2022 September;23:872. PubMed: 36127685 PMC: PMC9487131

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