Western blot of Collagen II (P133) pAb in extracts from COLO205 cell.
COL2A1 / Collagen II Alpha 1 Antibody (Pro133)
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human, Mouse, Rat |
| Format: | Liquid |
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- Description
- Collagen II Alpha 1 antibody LS-C175971 is an unconjugated rabbit polyclonal antibody to Collagen II Alpha 1 (COL2A1) (Pro133) from human. It is reactive with human, mouse and rat. Validated for IF, IHC and WB. Cited in 2 publications.
- Application
- IF, IHC, IHC-P, WB
- Reactivity
- Hu, Ms, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Alpha-1 type II collagen | Cartilage collagen | COL11A3 | Collagen alpha 1(ii) | Collagen, type II, alpha 1 | Collagen alpha-1(II) chain | Chondrocalcin | ANFH | AOM | COL2A1 | SEDC
- UniProt Number
- P02458
- NCBI GenBank
- NM_001844 | NP_001835.3
- SwissProt
- P02458
Target
- Target
- Human COL2A1 / Collagen II Alpha 1
- Antigen Species
- Human
- Epitope
- Human COL2A1
- Gene Name
- COL2A1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic peptide around Pro133 of human COL2A1
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.2, 0.05% Sodium Azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Human immunodeficiency virus type 1 enhancer-binding protein 3 is essential for the expression of asparagine-linked glycosylation 2 in the regulation of osteoblast and chondrocyte differentiation. Imamura K, Maeda S, Kawamura I, Matsuyama K, Shinohara N, Yahiro Y, Nagano S, Setoguchi T, Yokouchi M, Ishidou Y, Komiya S. The Journal of biological chemistry. 2014 Apr;289:9865-79.
PubMed: 24563464 · PMC: PMC3975031
CDC5L promotes early chondrocyte differentiation and proliferation by modulating pre-mRNA splicing of SOX9, COL2A1, and WEE1. Go Jokoji, Shingo Maeda, Kazuki Oishi, Toshiro Ijuin, Masahiro Nakajima, Hiroki Tawaratsumida, Ichiro Kawamura, Hiroyuki Tominaga, Eiji Taketomi, Shiro Ikegawa, Noboru Taniguchi. The Journal of biological chemistry. 2021 Aug;;297:100994.
PubMed: 34298017 · PMC: PMC8363834