LSBio
Polyclonal Rabbit anti-Human COL2A1 / Collagen II Alpha 1 Antibody (WB)

Western blot analysis of extracts of mouse eye, using COL2A1 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 30s.

Polyclonal Rabbit anti-Human COL2A1 / Collagen II Alpha 1 Antibody (WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Western Blot
Reactivity:Mouse
Format:Liquid
See all Human COL2A1 / Collagen II Alpha 1 antibodies →
$281.00each
Catalog Number: LS-C331539-20

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Overview

Description
Collagen II Alpha 1 antibody LS-C331539 is an unconjugated rabbit polyclonal antibody to mouse Collagen II Alpha 1 (COL2A1). Validated for WB.
Application
IHC, WB
Reactivity
Ms
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
Alpha-1 type II collagen | Cartilage collagen | COL11A3 | Collagen alpha 1(ii) | Collagen, type II, alpha 1 | Collagen alpha-1(II) chain | Chondrocalcin | ANFH | AOM | COL2A1 | SEDC
Recommended Dilution: WB
1:500 - 1:2000
UniProt Number
P02458
NCBI GenBank
NM_001844 | NP_001835.3
SwissProt
P02458

Target

Target
Human COL2A1 / Collagen II Alpha 1
Antigen Species
Human
Epitope
Human COL2A1 / Collagen II.
Gene Name
COL2A1

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1159-1418 of human COL2A1 (NP_149162.2). PREKGPDPLQYMRADQAAGGLRQHDAEVDATLKSLNNQIESIRSPEGSRKNPARTCRDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETGETCVYPNPANVPKKNWWSSKSKEKKHIWFGETINGGFHFSYGDDNLAPNTANVQMTFLRLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEIRAEGNSRFTYTALKDGCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGPEQEFGVDIGPVCFL
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Volume
20 Microliter
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.