Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
PLK1 / PLK-1 Antibody (clone OTI8C12)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Reactivity: | Dog, Human, Non-Human Primate |
| Format: | Liquid |
$443.00each
Catalog Number: LS-C114499-100
Catalog Number: LS-C114499
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- PLK-1 antibody LS-C114499 is an unconjugated mouse monoclonal antibody to PLK-1 (PLK1) from human. It is reactive with human, dog and monkey. Validated for Flow, IF, IP and WB.
- Application
- FC, IF, IP, WB
- Reactivity
- Can, Hu, NHP
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
- Recommended Dilution: FC
- 1:100
- Recommended Dilution: IF/ICC
- 1:100
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- P53350
- NCBI GenBank
- NM_005030 | NP_005021.2
- SwissProt
- P53350
Target
- Target
- Human PLK1 / PLK-1
- Antigen Species
- Human
- Epitope
- Human PLK / PLK1
- Gene Name
- PLK1
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- OTI8C12
- Isotype
- IgG1
Immunogen
- Immunogen
- Protein expressed in 293T cell transfected with human PLK1 expression vector.
- Immunogen Type
- Cell-Based Immunization
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
- Concentration
- 0.96 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Protein A/G Affinity
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS