Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-PLK1 antibody IHC of human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Human PLK1 / PLK-1 Antibody (phospho-Thr210, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B50-50
Catalog Number: LS-B50
For bulk orders or custom formulations:
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- Description
- PLK-1 antibody LS-B50 is an unconjugated rabbit polyclonal antibody to human PLK-1 (PLK1) (pThr210). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
- Recommended Dilution: ELISA
- 1:3000 - 1:12000
- Recommended Dilution: IHC-P
- 10 µg/ml
- Recommended Dilution: WB
- 1:200 - 1:2000
- UniProt Number
- P53350
- NCBI GenBank
- NM_005030 | NP_005021.2
- SwissProt
- P53350
Target
- Target
- PLK1 / PLK-1
- Antigen Species
- Human
- Specificity
- This antibody was affinity purified. This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Epitope
- This antibody was affinity purified. This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Gene Name
- PLK1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Mechanism of the interaction of beta(2)-glycoprotein I with negatively charged phospholipid membranes. Hammel M, Schwarzenbacher R, Gries A, Kostner GM, Laggner P, Prassl R. Biochemistry. Nov;40:14173-81. PubMed: 11714270
