LSBio
Monoclonal Mouse anti-Human PLK1 / PLK-1 Antibody (clone OTI3F12, IHC, WB)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

Monoclonal Mouse anti-Human PLK1 / PLK-1 Antibody (clone OTI3F12, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human PLK1 / PLK-1 antibodies →
$443.00each
Catalog Number: LS-C114491-100

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Overview

Description
PLK-1 antibody LS-C114491 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1). Validated for Flow, IHC, IP and WB. Cited in 2 publications.
Application
FC, IHC, IHC-P, IP, WB
Reactivity
Hu
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
PLK1 | Polo like kinase | Polo-like kinase (Drosophila) | Polo-like kinase 1 | STPK13 | Polo (Drosophia)-like kinase | PLK | PLK-1
Recommended Dilution: FC
1:100
Recommended Dilution: IHC-P
1:150
Recommended Dilution: WB
1:2000
UniProt Number
P53350
SwissProt
P53350

Target

Target
Human PLK1 / PLK-1
Antigen Species
Human
Epitope
Human PLK / PLK1
Gene Name
PLK1

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
OTI3F12
Isotype
IgG1

Immunogen

Immunogen
Protein expressed in 293T cell transfected with human PLK1 expression vector.
Immunogen Type
Cell-Based Immunization

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Concentration
1.01 mg/mL
Volume
100 Microliter
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS

Upregulation of FOXP1 is a new independent unfavorable prognosticator and a specific predictor of lymphatic dissemination in cutaneous melanoma patients. Donizy P, Pagacz K, Marczuk J, Fendler W, Maciejczyk A, Halon A, Matkowski R. OncoTargets and therapy. 2018 March;11:1413-1422.

PubMed: 29559799 · PMC: PMC5857151


Polo-like kinase-1 immunoreactivity is associated with metastases in cutaneous melanoma. Maciej Kaczorowski, Tomasz Borowiec, Piotr Donizy, Konrad Pagacz, Wojciech Fendler, Artur Lipinski, Agnieszka Halon, Rafal Matkowski. Journal of cutaneous pathology. 2017 October;44:819-826.

PubMed: 28626898

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