Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Anti-Pdcd4 Antibody - Immunohistochemistry. Antibody Anti-Pdcd4 pS457 (MOUSE) Monoclonal Antibody - 200-301-964 has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene. A representative image from positively stained small intestine shows the localization of the anti-Pdcd4 antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
PDCD4 Antibody (phospho-Ser457, clone 9G6)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Human, Mouse, Rat, Xenopus |
| Format: | Liquid |
$586.00each
Catalog Number: LS-C154004-100
Catalog Number: LS-C154004
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- PDCD4 antibody LS-C154004 is an unconjugated mouse monoclonal antibody to PDCD4 (pSer457) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Cited in 1 publication.
- Application
- ELISA, IHC, WB
- Reactivity
- Hu, Ms, Rt, Xen
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- H731 | Nuclear antigen H731-like | Nuclear antigen H731 | PDCD4 | Protein 197/15a
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC
- 1:1000 - 1:5000
- Recommended Dilution: WB
- 1:2000 - 1:10000
- UniProt Number
- Q53EL6
- NCBI GenBank
- NM_014456 | NP_055271.2
- SwissProt
- Q53EL6
Target
- Target
- Human PDCD4
- Antigen Species
- Human
- Epitope
- This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
- Gene Name
- PDCD4
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 9G6
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009;284:18515-24. PubMed: 19419954 PMC: PMC2709372
