Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-PDCD4 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC-plus™ Polyclonal Rabbit anti-Human PDCD4 Antibody (phospho-Ser457, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B487-50
Catalog Number: LS-B487
For bulk orders or custom formulations:
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- Description
- PDCD4 antibody LS-B487 is an unconjugated rabbit polyclonal antibody to PDCD4 (pSer457) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Predicted Reactivity
- Mouse, Rat, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- H731 | Nuclear antigen H731-like | Nuclear antigen H731 | PDCD4 | Protein 197/15a
- Recommended Dilution: ELISA
- 1:400000
- Recommended Dilution: IHC-P
- 1.25 - 2.5 µg/ml
- Recommended Dilution: WB
- 1:500 - 1:2000
- UniProt Number
- Q53EL6
- NCBI GenBank
- NM_014456 | NP_055271.2
- SwissProt
- Q53EL6
Target
- Target
- PDCD4
- Antigen Species
- Human
- Specificity
- This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
- Epitope
- This antibody is specific for human Pdcd4 protein phosphorylated at Ser457. A BLAST analysis was used to suggest cross-reactivity with Pdcd4 from human, mouse, rat and Xenopus based on 100% homology with the immunizing sequence. Cross-reactivity with Pdcd4 from other sources has not been determined.
- Gene Name
- PDCD4
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Isotype
- IgG
- Origin Species
- Human
Immunogen
- Immunogen
- Anti-Pdcd4 pS457 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Ser457 in the human Pdcd4 protein.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion. Huang TH, Wu F, Loeb GB, Hsu R, Heidersbach A, Brincat A, Horiuchi D, Lebbink RJ, Mo YY, Goga A, McManus MT. The Journal of biological chemistry. 2009;284:18515-24. PubMed: 19419954 PMC: PMC2709372
