Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-GPR30 antibody IHC staining of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PathPlus™ Polyclonal Rabbit anti-Human GPER1 / GPR30 Antibody (Cytoplasmic Domain, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$449.00each
Catalog Number: LS-A1183-50
Catalog Number: LS-A1183
For bulk orders or custom formulations:
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- Description
- GPR30 antibody LS-A1183 is an unconjugated rabbit polyclonal antibody to human GPR30 (GPER1) (Cytoplasmic Domain). Validated for ICC, IF, IHC and WB. Cited in 4 publications.
- Application
- IHC
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CMKRL2 | CEPR | FEG-1 | G-protein coupled receptor 30 | G protein-coupled receptor 30 | GPCR-Br | GPER | Heptahelix receptor | LERGU | LyGPR | MER | GPR30 | IL8-related receptor DRY12 | LERGU2 | Chemokine receptor-like 2 | DRY12 | Membrane estrogen receptor
- Recommended Dilution: IHC-P
- 3 - 4 µg/ml
- UniProt Number
- Q99527
- NCBI GenBank
- NM_001505 | NP_001496.1
- SwissProt
- Q99527
Target
- Target
- GPER1 / GPR30
- Antigen Species
- Human
- Specificity
- Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Epitope
- Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Gene Name
- GPER1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Marmoset (94%); Monkey, Panda (88%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Identity of an estrogen membrane receptor coupled to a G protein in human breast cancer cells. Thomas P, Pang Y, Filardo EJ and Dong J. Endocrinology. 2005;146:624-32.
PubMed: 15539556
The G protein-coupled receptor GPR30 mediates the proliferative effects induced by 17beta-estradiol and hydroxytamoxifen in endometrial cancer cells. Vivacqua A, Bonofiglio D, Recchia AG, Musti AM, Picard D, Andò S and Maggiolini M. Molecular endocrinology (Baltimore, Md.). 2006;20:631-46.
PubMed: 16239258 · PMC: PMC3060528
Estrogenic GPR30 signalling induces proliferation and migration of breast cancer cells through CTGF. Pandey DP, Lappano R, Albanito L, Madeo A, Maggiolini M, Picard D. The EMBO journal. 2009;28:523-32.
PubMed: 19153601 · PMC: PMC2657575
Inhibition of the AKT/mTOR and erbB pathways by gefitinib, perifosine and analogs of gonadotropin-releasing hormoneI andII to overcome tamoxifen resistance in breast cancer cells. Block M, Gr[Character fc]ndker C, Fister S, Kubin J, Wilkens L, Mueller MD, Hemmerlein B, Emons G, G[Character fc]nthert AR. International journal of oncology. 2012 Nov;41:1845-54.
PubMed: 22922893
