Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-GPR30 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
PathPlus™ Polyclonal Rabbit anti-Human GPER1 / GPR30 Antibody (C-Terminus, IHC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Dog, Human, Non-Human Primate |
| Format: | Liquid |
$449.00each
Catalog Number: LS-A4272-50
Catalog Number: LS-A4272
For bulk orders or custom formulations:
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- Description
- GPR30 antibody LS-A4272 is an unconjugated rabbit polyclonal antibody to GPR30 (GPER1) (C-Terminus) from human. It is reactive with human, rat, dog and other species. Validated for IF, IHC and WB. Cited in 9 publications.
- Application
- IHC
- Reactivity
- Can, Hu, NHP
- Predicted Reactivity
- Horse, Rat
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- CMKRL2 | CEPR | FEG-1 | G-protein coupled receptor 30 | G protein-coupled receptor 30 | GPCR-Br | GPER | Heptahelix receptor | LERGU | LyGPR | MER | GPR30 | IL8-related receptor DRY12 | LERGU2 | Chemokine receptor-like 2 | DRY12 | Membrane estrogen receptor
- Recommended Dilution: IHC-P
- 5 - 10 µg/ml
- UniProt Number
- Q99527
- NCBI GenBank
- NM_001505 | NP_001496.1
- SwissProt
- Q99527
Target
- Target
- GPER1 / GPR30
- Antigen Species
- Human
- Specificity
- Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Epitope
- Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Gene Name
- GPER1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic 18 amino acid peptide from C-Terminus of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Dog (100%); Rat, Horse (94%); Mouse, Hamster (89%); Elephant, Bovine (83%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Impaired leukocyte influx in cervix of postterm women not responding to prostaglandin priming. Sahlin L, Stjernholm-Vladic Y, Roos N, Masironi B, Ekman-Ordeberg G. Reproductive biology and endocrinology : RB&E. 2008;6:36.
PubMed: 18764934 · PMC: PMC2551600
Spinal synthesis of estrogen and concomitant signaling by membrane estrogen receptors regulate spinal - and -opioid receptor heterodimerization and female-specific spinal morphine antinociception. Liu NJ, Chakrabarti S, Schnell S, Wessendorf M, Gintzler AR. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011;31:11836-45.
PubMed: 21849544 · PMC: PMC3321927
Regulation of ERRalpha gene expression by estrogen receptor agonists and antagonists in SKBR3 breast cancer cells: differential molecular mechanisms mediated by g protein-coupled receptor GPR30/GPER-1. Li Y, Birnbaumer L, Teng CT. Molecular endocrinology (Baltimore, Md.). 2010 May;24:969-80.
PubMed: 20211987 · PMC: PMC2870941
Stallion spermatozoa: putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER. Arkoun B, Gautier C, Delalande C, Barrier-Battut I, Gu[Character e9]non I, Goux D, Boura[Character ef]ma-Lelong H. General and comparative endocrinology. 2014 May;200:35-43.
PubMed: 24607572
Estradiol and GPER Activation Differentially Affect Cell Proliferation but Not GPER Expression in the Hippocampus of Adult Female Rats. Duarte-Guterman P, Lieblich SE, Chow C, Galea LA. PloS one. 2015;10:e0129880.
PubMed: 26075609 · PMC: PMC4468121
GPR30 regulates the EGFR-Akt cascade and predicts lower survival in patients with ovarian cancer. Fujiwara S, Terai Y, Kawaguchi H, Takai M, Yoo S, Tanaka Y, Tanaka T, Tsunetoh S, Sasaki H, Kanemura M, Tanabe A, Yamashita Y, Ohmichi M. Journal of ovarian research. 2012;5:35.
PubMed: 23163984
G protein-coupled receptor 30 localizes to the endoplasmic reticulum and is not activated by estradiol. Otto C, Rohde-Schulz B, Schwarz G, Fuchs I, Klewer M, Brittain D, Langer G, Bader B, Prelle K, Nubbemeyer R, Fritzemeier KH. Endocrinology. 2008;149:4846-56.
PubMed: 18566127
Severe pulmonary hypertension in aging female apolipoprotein E-deficient mice is rescued by estrogen replacement therapy. Umar S, Partow-Navid R, Ruffenach G, Iorga A, Moazeni S, Eghbali M. Biology of sex differences. 2017 March;8:9.
PubMed: 28344760 · PMC: PMC5360087
A genetic polymorphism repurposes the G-protein coupled and membrane-associated estrogen receptor GPER to a transcription factor-like molecule promoting paracrine signaling between stroma and breast carcinoma cells. Pupo M, Bodmer A, Berto M, Maggiolini M, Dietrich PY, Picard D. Oncotarget. 2017 July;8:46728-46744.
PubMed: 28596490 · PMC: PMC5564519
