Western blot of NOD2 using Polyclonal Antibody to NOD2/CARD15 at 1:2000.30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.
NOD2 / CARD15 Antibody (aa582-600)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$394.00each
Catalog Number: LS-C148280-50
Catalog Number: LS-C148280
For bulk orders or custom formulations:
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- Description
- CARD15 antibody LS-C148280 is an unconjugated rabbit polyclonal antibody to human CARD15 (NOD2) (aa582-600). Validated for IHC, IP and WB.
- Application
- IHC, IHC-F, IHC-P, IP, WB
- Reactivity
- Hu
- Predicted Reactivity
- Non-Human Primate
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- BLAU | CARD15 | ACUG | NOD-like receptor C2 | NOD2B | NLRC2 | IBD1 | NOD2 | CLR16.3 | PSORAS1
- Recommended Dilution: IHC-P
- 1:1000 - 1:5000
- Recommended Dilution: IP
- 1:50 - 1:200
- Recommended Dilution: WB
- 1:1000 - 1:2000
- UniProt Number
- Q9HC29
- NCBI GenBank
- NM_022162 | NP_071445.1
- SwissProt
- Q9HC29
Target
- Target
- Human NOD2 / CARD15
- Antigen Species
- Human
- Epitope
- Human NOD2.
- Gene Name
- NOD2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- A synthetic peptide corresponding to amino acids 582-600 (LGFLVRAKGVVPGSTAPLE) of human NOD2/CARD15. NOD2 is a 1040 amino acid protein. The immunogen sequence is 100% conserved in certain NOD2 isoforms and mutations. Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon (100%); Monkey (95%); Gorilla, Orangutan, Sheep, Zebu, Bovine, Rabbit (89%); Tamarin, Rat, Elephant, Pig (84%); Mouse (79%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.05% sodium azide
- Volume
- 50 Microliter
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.