Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NF-L / NEFL antibody (lane 4).
NF-L / NEFL Antibody (clone DA2)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Western Blot |
| Reactivity: | Bovine, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204554-100
Catalog Number: LS-C204554
For bulk orders or custom formulations:
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- Description
- NEFL antibody LS-C204554 is an unconjugated mouse monoclonal antibody to NEFL (NF-L) from pig. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
- Application
- ICC, IF, WB
- Reactivity
- Bov, Hu, Ms, Por, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 68 kDa neurofilament protein | CMT1F | CMT2E | NEFL | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
- Recommended Dilution: IF/ICC
- 1:500 - 1:1000|0.388888888888889 - 1:1000
- Recommended Dilution: WB
- 1:5000
- UniProt Number
- P07196
- NCBI GenBank
- NM_006158 | NP_006149.2
- SwissProt
- P07196
Target
- Target
- Pig NF-L / NEFL
- Antigen Species
- Pig
- Epitope
- Reacts with NF-L from human, cow, pig, mouse, rat and all other mammals, and also recognizes avian NF-L. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.
- Gene Name
- NEFL
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- DA2
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Preparation of enzymatically dephosphorylated pig neurofilaments including NF-L, NF-M and NF-H.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 50% PBS, 50% glycerol plus 5mM NaN3
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS