Strip blots of rat spinal cord homogenate probed with two subclones of NF-L / NEFL antibody. Both subclones, one of which became the definitive antibody, stain strongly and specifically a band at 68 kDa with essentially no background.
NF-L / NEFL Antibody (clone 7D1)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Western Blot |
| Reactivity: | Bovine, Human, Mouse, Pig, Rat |
| Format: | Liquid |
$384.00each
Catalog Number: LS-C204557-100
Catalog Number: LS-C204557
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- Description
- NEFL antibody LS-C204557 is an unconjugated mouse monoclonal antibody to NEFL (NF-L) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF and WB.
- Application
- ICC, IF, IHC, WB
- Reactivity
- Bov, Hu, Ms, Por, Rt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- 68 kDa neurofilament protein | CMT1F | CMT2E | NEFL | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
- Recommended Dilution: IF/ICC
- 1:100 - 1:500|0.111111111111111 - 1:500
- Recommended Dilution: WB
- 1:5000 - 1:10000
- UniProt Number
- P07196
- NCBI GenBank
- NM_006158 | NP_006149.2
- SwissProt
- P07196
Target
- Target
- Human NF-L / NEFL
- Antigen Species
- Human
- Epitope
- Reacts with NF-L from human, cow, pig, mouse, rat and all other mammals. It is strong and clean on western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections. The epitope is not fully characterized since it could not be localized using 20 amino acid nested peptides but is known to be not within the a-helical region, amino acids 88-400 of the human protein.
- Gene Name
- NEFL
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 7D1
- Isotype
- IgG2b
Immunogen
- Immunogen
- Preparation of pig neurofilament NF-H and NF-L.
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 50% PBS, 50% glycerol, plus 5 mM sodium azide
- Concentration
- 1 mg/mL
- Volume
- 100 Microliter
- Preservative
- Yes
- Purification Method
- Other
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS