Anti-NF-L / NEFL antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ NF-L / NEFL Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Chicken Polyclonal
Applications:	Immunocytochemistry, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human
Format:	Liquid

$383.00each

Catalog Number: LS-B10376-50

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Overview

Description: NEFL antibody LS-B10376 is an unconjugated chicken polyclonal antibody to human NEFL (NF-L). Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: ICC, IF, IHC, IHC-P, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: 68 kDa neurofilament protein | CMT1F | CMT2E | NEFL | NF68 | NF-L | NFL | Neurofilament subunit NF-L | PPP1R110 | Neurofilament light polypeptide | CMTDIG
Recommended Dilution: IF/ICC: 1:5000
Recommended Dilution: IHC-P: 1:500
Recommended Dilution: WB: 1:10000
UniProt Number: P07196
NCBI GenBank: NM_006158 | NP_006149.2
SwissProt: P07196

Target

Target: NF-L / NEFL
Antigen Species: Human
Epitope: Human NF-L / NEFL
Gene Name: NEFL

Antibody

Host Species: Chicken
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: To raise this antibody bovine intermediate filaments were prepared from spinal cords by the method of Delacourte et al. and the cytoskeletal material was dissolved in 6M urea. Individual neurofilament subunits were purified by ion exchange chromatography on DEAE cellulose followed by preparative gel electrophoresis. To ensure greater specificity for NF-L, animals were boosted with recombinant mouse NF-L purified from bacteria.