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Monoclonal Mouse anti-Human TP53 / p53 Antibody (clone DO-7, aa20-25, IHC, WB)

Monoclonal Mouse anti-Human TP53 / p53 Antibody (clone DO-7, aa20-25, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Flow Cytometry, Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Human
Format:Liquid
See all Human TP53 / p53 antibodies →
$296.00each
Catalog Number: LS-C11556-0.1

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Overview

Description
P53 antibody LS-C11556 is an unconjugated mouse monoclonal antibody to human p53 (TP53) (aa20-25). Validated for Flow, IHC, IP and WB.
Application
FC, IHC-F, IHC-P, IP, WB
Reactivity
Hu
Nonreactive Species
Mouse, Rat
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Recommended Dilution: FC
1:50 - 1:100
Recommended Dilution: IHC-P
1:1000
Recommended Dilution: WB
1:1000
UniProt Number
P04637
NCBI GenBank
NM_000546 | NP_000537.3
SwissProt
P04637

Target

Target
Human TP53 / p53
Antigen Species
Human
Epitope
Recognizes the human 53kD p53 tumor suppressor protein, also known as Cellular tumor antigen p53 or Antigen NY-CO-13, encoded by the TP53 gene. p53 is a 393 amino acid protein with an N-terminal transactivation domain, followed by a proline-rich region and a DNA binding domain in the central core region. The C-terminal region contains a tetramerization domain and a terminal regulatory domain. p53 is intimately involved in a number of signaling pathways controlling cell division, cycling and apoptosis and is thus a potent cancer suppressor. In normal cells the level of p53 expression is low but can be induced by DNA damage or other stress signals. Activation of p53 leads to growth arrest through its interaction with p21, GADD45 and, DNA repair and potentially apoptosis through interaction with, and. p53 is critically regulated by Mdm2 which can trigger p53 degradation by a ubiquitin dependent systemrecognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. Clone DO-7 is not expected to recognize the multiple lacking the N-terminal region.
Gene Name
TP53

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
DO-7
Isotype
IgG2b

Immunogen

Immunogen
Recombinant human p53 (AA 20-25)
Immunogen Type
Recombinant Protein Fragment

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.09% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Recommended Storage Buffer
PBS

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