Anti-TP53 / p53 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ TP53 / p53 Antibody (clone BP53-12)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Chromatin Immunoprecipitation, Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:	Human
Format:	Liquid

$526.00each

Catalog Number: LS-B431-50

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Overview

Description: P53 antibody LS-B431 is an unconjugated mouse monoclonal antibody to human p53 (TP53). Validated for ChrIP, ELISA, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Application: ChIP, ELISA, IF, IHC, IHC-P, IP, WB
Reactivity: Hu
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | TP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Recommended Dilution: ELISA: 1:2000 - 1:10000
Recommended Dilution: IF/ICC: 1:100 - 1:500
Recommended Dilution: IHC-P: 2.5 µg/ml
Recommended Dilution: IP: 1:100; 1 µg/µl
Recommended Dilution: WB: 1:500 - 1:2000
UniProt Number: P04637
NCBI GenBank: NM_000546 | NP_000537.3
SwissProt: P04637

Target

Target: TP53 / p53
Antigen Species: Human
Specificity: Reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
Epitope: Reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
Gene Name: TP53

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: BP53-12
Light Chain: Kappa Light Chain
Origin Species: Human

Immunogen

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.5 M NaCl

Cancer. p53, guardian of the genome. Lane DP. Nature. 1992;358:15-6.

PubMed: 1614522

p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B, Harris CC. Science (New York, N.Y.). 1991;253:49-53.

PubMed: 1905840

Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. Gurney EG, Harrison RO, Fenno J. Journal of virology. 1980;34:752-63.

PubMed: 6155477 · PMC: PMC288763

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