Monoclonal Hamster anti-Mouse NOTCH2 Antibody (clone HMN2-35)
Primary AntibodyLSBio Guarantee
| Antibody: | Hamster Monoclonal |
| Applications: | Flow Cytometry, Immunohistochemistry (general) |
| Reactivity: | Mouse |
| Format: | Liquid |
$415.00each
Catalog Number: LS-C188663-0.25
Catalog Number: LS-C188663
For bulk orders or custom formulations:
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- Description
- NOTCH2 antibody LS-C188663 is an unconjugated hamster monoclonal antibody to mouse NOTCH2. Validated for Flow.
- Application
- FC, IHC
- Reactivity
- Ms
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- AGS2 | HN2 | Notch (Drosophila) homolog 2 | Notch homolog 2 (Drosophila) | Notch 2 | Notch homolog 2 | NOTCH2 | HJCYS
- UniProt Number
- Q04721
- NCBI GenBank
- NM_024408 | NP_077719.2
- SwissProt
- Q04721
Target
- Target
- Mouse NOTCH2
- Antigen Species
- Mouse
- Epitope
- Recognizes Notch 2, one of the four major transmembrane receptors (Notch 1-4) of the Notch signalling pathway, which is activated through binding to DSL domain-containing membrane-bound specific ligands. The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumor progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Signalling through Notch 2 has been implicated in the development of marginal zone B cells (MZB), the sensitization of endothelial cells to apoptosis, and the regulation of the expression of CD23 in B-cell lymphocytic leukaemia (B-CLL). Studies have also shown a correlation between a decrease in Notch 2 expression and an increase in grade of human breast cancer. Clone HMN2-35 has been shown to cross-react with rat mast cell line RBL-2H3 and Y3 myeloma cells, in flow cytometry.
- Gene Name
- NOTCH2
Antibody
- Host Species
- Hamster
- Clonality
- Monoclonal
- Clone
- HMN2-35
- Isotype
- IgG
Immunogen
- Immunogen
- Mouse Notch 2-Fc fusion protein.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.09% Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein G Affinity
Storage & Handling
- Storage Conditions
- Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
- Recommended Storage Buffer
- PBS
