Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-NOTCH2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC-plus™ Polyclonal Rabbit anti-Human NOTCH2 Antibody (N-Terminus, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B719-50
Catalog Number: LS-B719
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- Description
- NOTCH2 antibody LS-B719 is an unconjugated rabbit polyclonal antibody to human NOTCH2 (N-Terminus). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- AGS2 | HN2 | Notch (Drosophila) homolog 2 | Notch homolog 2 (Drosophila) | Notch 2 | Notch homolog 2 | NOTCH2 | HJCYS
- Recommended Dilution: ELISA
- 1:20000 - 1:100000
- Recommended Dilution: IHC-P
- 1:500
- Recommended Dilution: WB
- 1:400 - 1:2000
- UniProt Number
- Q04721
- NCBI GenBank
- NM_024408 | NP_077719.2
- SwissProt
- Q04721
Target
- Target
- NOTCH2
- Antigen Species
- Human
- Specificity
- This antiserum is directed against human NOTCH2. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.
- Epitope
- This antiserum is directed against human NOTCH2. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form. Based on sequences, we expect this antibody to react as well with rat and mouse NOTCH 2. Only one amino acid difference (conservative) occurs in mouse and rat.
- Gene Name
- NOTCH2
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 2 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Volume
- 50 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate, 0.15 M NaCl
Notch signaling pathway in cumulus cells can be a novel marker to identify poor and normal responder IVF patients. Tanriverdi G, Denir S, Ayla S, Bilir A, Oktar H, Cepni I, Irez T. Journal of assisted reproduction and genetics. 2013 Oct;30:1319-26. PubMed: 23922013 PMC: PMC3824856
