Anti-NOTCH2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

PathPlus™ NOTCH2 Antibody (aa2390-2415)

Primary AntibodyLSBio Guarantee

Antibody:	Rabbit Polyclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Dog, Human, Non-Human Primate
Format:	Liquid

$526.00each

Catalog Number: LS-B399-50

Unit Size

Quantity

For bulk orders or custom formulations:

Overview

Description: NOTCH2 antibody LS-B399 is an unconjugated rabbit polyclonal antibody to NOTCH2 (aa2390-2415) from human. It is reactive with human, chimpanzee and dog. Validated for ELISA, IHC and WB. Cited in 3 publications.
Application: ELISA, IHC, IHC-P, WB
Reactivity: Can, Hu, NHP
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names: AGS2 | HN2 | Notch (Drosophila) homolog 2 | Notch homolog 2 (Drosophila) | Notch 2 | Notch homolog 2 | NOTCH2 | HJCYS
UniProt Number: Q04721
NCBI GenBank: NM_024408 | NP_077719.2
SwissProt: Q04721

Recommended Dilution

Application	Dilution
ELISA	1:30000 - 1:90000
IHC-P	1:500
WB	1:400 - 1:2000

Target

Target: NOTCH2
Antigen Species: Human
Specificity: This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
Epitope: This antiserum is directed against human NOTCH 2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse NOTCH 2. No specific information is available for other reactivities.
Gene Name: NOTCH2

Antibody

Host Species: Rabbit
Clonality: Polyclonal
Origin Species: Human

Immunogen

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type: Synthetic Peptide (Linear)

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Concentration: 75 mg/mL
Volume: 50 Microliter
Animal Free: No
Carrier-Free: Yes
Preservative: Yes

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl

Differential expression of Notch receptors and their ligands in desmoplastic ameloblastoma. Siar CH, Nakano K, Han PP, Nagatsuka H, Ng KH, Kawakami T. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2010;39:552-8.

PubMed: 20337864

Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor. Siar CH, Ha KO, Aung LO, Nakano K, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T. European journal of medical research. 2010;15:456-60.

PubMed: 21156405 · PMC: PMC3352190

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