Anti-SR Proteins antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ SR Proteins Antibody

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Bovine, Human, Mouse, Rabbit, Rat
Format:	Liquid

$495.00each

Catalog Number: LS-B5510-50

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Overview

Description: SR Proteins antibody LS-B5510 is an unconjugated mouse monoclonal antibody to SR Proteins from bovine. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Application: ELISA, IF, IHC, IHC-P, WB
Reactivity: Bov, Hu, Ms, Rb, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Recommended Dilution: ELISA: 0.1 - 1 µg/ml
Recommended Dilution: IF/ICC: 0.5 - 2 µg/ml
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: WB: 3 - 5 µg/ml

Target

Target: SR Proteins
Antigen Species: Bovine
Specificity: Detects a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species cross-reactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.
Epitope: Detects a sub-set of the non-snRNP splicing factors termed SR proteins. The antibody detects SRp75, SRp55, SRp40 and SRp20 but not SRp30a or b (ASF/SF2, SC-35) proteins. Recognizes approximately 20 distinct nuclear proteins including the U1 70K component of the U1 snRNP and both subunits of U2AF1. Species cross-reactivity (Western Blot): chicken, human, mouse, rabbit, canine, rat, Xenopus. Reactivity has not been detected with yeasts.

Antibody

Host Species: Mouse
Clonality: Monoclonal
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Purified, de-phosphorylated bovine Srp55 and Drosophila p55-GST.

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, pH 7.2, 0.1% Sodium Azide
Concentration: 0.5 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Protein A Affinity

Storage & Handling

Storage Conditions: May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for up to 1 year.
Recommended Storage Buffer: PBS