SMO / Smoothened Antibody
Catalog Number: LS-C408690
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Human Kidney: Formalin-Fixed Paraffin-Embedded (FFPE)
IHC-plus™ SMO / Smoothened Antibody (N-Terminus)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Bovine, Dog, Hamster, Horse, Human, Mammal (Other), Mouse, Non-Human Primate, Pig, Rabbit, Rat |
| Format: | Liquid |
$449.00each
Catalog Number: LS-A2666-50
Catalog Number: LS-A2666
For bulk orders or custom formulations:
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- Description
- Smoothened antibody LS-A2666 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (N-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
- Application
- IHC
- Reactivity
- Bov, Can, Ham, Hr, Hu, Mml, Ms, NHP, Por, Rb, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | SMO | Smoothened homolog
- Recommended Dilution: IHC-P
- 10 µg/ml
- UniProt Number
- Q99835
- NCBI GenBank
- NM_005631 | NP_005622.1
- SwissProt
- Q99835
Target
- Target
- SMO / Smoothened
- Antigen Species
- Human
- Specificity
- N-terminus of SMO from human, mouse, rat and other mammals. BLAST analysis of the peptide immunogen showed no homology with other human proteins
- Epitope
- Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Gene Name
- SMO
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic 15 amino acid peptide from N-Terminus extracellular domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Platypus (100%); Opossum (93%); Turkey, Chicken, Xenopus (87%); Zebrafish, Seq squirt (80%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% Sodium Azide
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005;437:1018-21.
PubMed: 16136078
Sonic and desert hedgehog signaling in human fetal prostate development. Zhu G, Zhau HE, He H, Zhang L, Shehata B, Wang X, Cerwinka WH, Elmore J, He D. The Prostate. 2007 May;67:674-84.
PubMed: 17342747
Primary cilia modulate Ihh signal transduction in response to hydrostatic loading of growth plate chondrocytes. Shao YY, Wang L, Welter JF, Ballock RT. Bone. 2012 Jan;50:79-84.
PubMed: 21930256 · PMC: PMC3246537
Divergence of hedgehog signal transduction mechanism between Drosophila and mammals. Varjosalo M, Li SP, Taipale J. Developmental cell. 2009 Feb;10:177-86.
PubMed: 16459297
Primary cilia on porcine testicular somatic cells and their role in hedgehog signaling and tubular morphogenesis in vitro. Dores C, Alpaugh W, Su L, Biernaskie J, Dobrinski I. Cell and tissue research. 2017 April;368:215-223.
PubMed: 27841005 · PMC: PMC5495557
A role for primary cilia in aortic valve development and disease. Toomer KA, Fulmer D, Guo L, Drohan A, Peterson N, Swanson P, Brooks B, Mukherjee R, Body S, Lipschutz JH, Wessels A, Norris RA. Developmental dynamics : an official publication of the American Association of Anatomists. 2017 August;246:625-634.
PubMed: 28556366 · PMC: PMC5548133
Sonic hedgehog regulation of human rhabdosphincter muscle:Potential implications for treatment of stress urinary incontinence. Hehemann M, Kalmanek E, Choe S, Dynda D, Hu WY, Quek ML, Harrington DA, Stupp SI, McVary KT, Podlasek CA. Neurourology and urodynamics. 2018 November;37:2551-2559.
PubMed: 30187971 · PMC: PMC6241312
Rapamycin treatment correlates changes in primary cilia expression with cell cycle regulation in epithelial cells. Maha H Jamal, Ane C F Nunes, Nosratola D Vaziri, Ramani Ramchandran, Robert L Bacallao, Andromeda M Nauli, Surya M Nauli. Biochemical pharmacology. 2020 August;178:114056.
PubMed: 32470549 · PMC: PMC7899243
