Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Ovary, Carcinoma
IHC-plus™ Polyclonal Rabbit anti-Human SMO / Smoothened Antibody (1st Extracellular Domain, IHC, IF)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general) |
| Reactivity: | Human |
| Format: | Liquid |
$403.00each
Catalog Number: LS-A2668-50
Catalog Number: LS-A2668
For bulk orders or custom formulations:
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- Description
- Smoothened antibody LS-A2668 is an unconjugated rabbit polyclonal antibody to Smoothened (SMO) (Cytoplasmic Domain) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
- Application
- IHC
- Reactivity
- Hu
- Predicted Reactivity
- Bovine, Dog, Chicken, Hamster, Horse, Mammal (Other), Mouse, Non-Human Primate, Pig, Rabbit, Rat, Xenopus
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- Frizzled family member 11 | FZD11 | Gx | Protein Gx | SMOH | Smoothened | SMO | Smoothened homolog
- Recommended Dilution: IHC-P
- 5 - 10 µg/ml
- UniProt Number
- Q99835
- NCBI GenBank
- NM_005631 | NP_005622.1
- SwissProt
- Q99835
Target
- Target
- SMO / Smoothened
- Antigen Species
- Human
- Specificity
- 1st Extracellular Domain of human, gibbon SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins
- Epitope
- Human SMO. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Gene Name
- SMO
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
Immunogen
- Immunogen
- Synthetic 16 amino acid peptide from 1st cytoplasmic domain of human SMO. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (100%); Marmoset, Mouse, Rat, Bovine, Dog, Bat, Hamster, Elephant, Panda, Horse, Rabbit, Pig, Turkey, Chicken, Platypus, Xenopus (94%); Opossum (88%).
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.1% Sodium Azide
- Concentration
- 1 mg/mL
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Vertebrate Smoothened functions at the primary cilium. Corbit KC, Aanstad P, Singla V, Norman AR, Stainier DY and Reiter JF. Nature. 2005;437:1018-21.
PubMed: 16136078
Basal cell carcinomas arise from hair follicle stem cells in Ptch1(+/-) mice. Wang GY, Wang J, Mancianti ML, Epstein EH Jr. Cancer cell. 2011;19:114-24.
PubMed: 21215705 · PMC: PMC3061401
Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines. Nielsen SK, M[Character f8]llg[Character e5]rd K, Clement CA, Veland IR, Awan A, Yoder BK, Novak I, Christensen ST. Developmental dynamics : an official publication of the American Association of Anatomists. 2008 Aug;237:2039-52.
PubMed: 18629868
Ptch2 shares overlapping functions with Ptch1 in Smo regulation and limb development. Zhulyn O, Nieuwenhuis E, Liu YC, Angers S, Hui CC. Developmental biology. Jan;397:191-202.
PubMed: 25448692
Mesothelial mobilization in the developing lung and heart differs in timing, quantity, and pathway dependency. Timo H Lüdtke, Carsten Rudat, Jennifer Kurz, Regine Häfner, Franziska Greulich, Irina Wojahn, Nurullah Aydogdu, Tamrat M Mamo, Marc-Jens Kleppa, Mark-Oliver Trowe, Tobias Bohnenpoll, Makoto Mark Taketo, Andreas Kispert. American journal of physiology. Lung cellular and molecular physiology. 2019 May;316:L767-L783.
PubMed: 30702346
