LSBio
IHC-plus™ RELA / NFKB p65 Antibody (C-Terminus)

Anti-RELA antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ RELA / NFKB p65 Antibody (C-Terminus)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Chromatin Immunoprecipitation, Immunohistochemistry (general)
Reactivity:Human, Mouse, Rat
Format:Liquid
See all RELA / NFKB p65 antibodies →
$526.00each
Catalog Number: LS-B653-50

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Overview

Description
NFKB p65 antibody LS-B653 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (C-Terminus) from human. It is reactive with human, mouse and rat. Validated for ChrIP, ELISA, GS, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 9 publications.
Application
ChIP, IHC
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Recommended Dilution: ELISA
1:5000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: WB
1:2000 - 1:5000
UniProt Number
Q04206
SwissProt
Q04206

Target

Target
RELA / NFKB p65
Antigen Species
Human
Gene Name
RELA

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.01% Sodium Azide
Concentration
80 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Other

Storage & Handling

Storage Conditions
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

Dexamethasone attenuates NF-kappa B DNA binding activity without inducing I kappa B levels in rat brain in vivo. Unlap MT, Jope RS. Brain research. Molecular brain research. 1997;45:83-9.

PubMed: 9105673


Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. Jobin C, Bradham CA, Russo MP, Juma B, Narula AS, Brenner DA, Sartor RB. Journal of immunology (Baltimore, Md. : 1950). 1999;163:3474-83.

PubMed: 10477620


Upregulation of HMG1 leads to melanoma inhibitory activity expression in malignant melanoma cells and contributes to their malignancy phenotype. Poser I, Golob M, Buettner R, Bosserhoff AK. Molecular and cellular biology. 2003;23:2991-8.

PubMed: 12665595 · PMC: PMC152547


NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. Cao S, Zhang X, Edwards JP, Mosser DM. The Journal of biological chemistry. 2006;281:26041-50.

PubMed: 16835236 · PMC: PMC2642587


Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. Lou H, Kaplowitz N. The Journal of biological chemistry. 2007;282:29470-81.

PubMed: 17690092


CXCL5 promotes prostate cancer progression. Begley LA, Kasina S, Mehra R, Adsule S, Admon AJ, Lonigro RJ, Chinnaiyan AM, Macoska JA. Neoplasia (New York, N.Y.). 2008;10:244-54.

PubMed: 18320069 · PMC: PMC2262133


Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. Yoon C, Korade Z, Carter BD. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2008;28:3738-46.

PubMed: 18385332


Axonal neuregulin 1 type III activates NF-kappaB in Schwann cells during myelin formation. Limpert AS, Carter BD. The Journal of biological chemistry. 2010;285:16614-22.

PubMed: 20360002 · PMC: PMC2878070


Nuclear factor-B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. Ghosh M, Yang Y, Rothstein JD, Robinson MB. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011;31:9159-69.

PubMed: 21697367 · PMC: PMC3138498

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