LSBio
IHC-plus™ Polyclonal Rabbit anti-Human RELA / NFKB p65 Antibody (phospho-Ser529, IHC, WB)

Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.

IHC-plus™ Polyclonal Rabbit anti-Human RELA / NFKB p65 Antibody (phospho-Ser529, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all RELA / NFKB p65 antibodies →
$526.00each
Catalog Number: LS-B652-50

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Overview

Description
NFKB p65 antibody LS-B652 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer529). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 6 publications.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Intended Use
RUO
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Recommended Dilution: ELISA
1:5000 - 1:25000
Recommended Dilution: IHC-P
1:500
Recommended Dilution: WB
1:500 - 1:3000
UniProt Number
Q04206
SwissProt
Q04206

Target

Target
RELA / NFKB p65
Antigen Species
Hu
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS529 phosphorylated p65 from mouse, rat or other species has not been determined.
Epitope
This phospho specific polyclonal antibody is specific for phosphorylated pS529 human p65. Reactivity with non-phosphorylated p65 is minimal. Cross reactivity with pS529 phosphorylated p65 from mouse, rat or other species has not been determined.
Gene Name
RELA

Antibody

Host Species
Rb
Clonality
Polyclonal
Origin Species
Hu

Immunogen

Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Format

Conjugate/Label
Unconj
Format
Liquid
Formulation
Antiserum, 0.1% sodium azide
Concentration
75 mg/mL
Volume
50 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Crude

Storage & Handling

Storage Conditions
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002;169:6490-7. PubMed: 12444159

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