LSBio
IHC-plus™ Polyclonal Rabbit anti-Human RELA / NFKB p65 Antibody (phospho-Ser536, IHC, WB)

Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Polyclonal Rabbit anti-Human RELA / NFKB p65 Antibody (phospho-Ser536, IHC, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all RELA / NFKB p65 antibodies →
$526.00each
Catalog Number: LS-B53-50

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Overview

Description
NFKB p65 antibody LS-B53 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer536). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application
ELISA, IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Mouse, Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Recommended Dilution: ELISA
1:1000 - 1:6000
Recommended Dilution: IHC-P
5 µg/ml
Recommended Dilution: WB
1:200 - 1:2000
UniProt Number
Q04206
SwissProt
Q04206

Target

Target
RELA / NFKB p65
Antigen Species
Human
Specificity
This antibody is specific for human p65 (RelA) protein phosphorylated at S536. A BLAST analysis was used to suggest cross reactivity with p65 (RelA) from human, mouse and rat based on 100% homology with the immunizing sequence. Cross reactivity with p65 (RelA) from other sources has not been determined.
Epitope
This antibody is specific for human p65 (RelA) protein phosphorylated at S536. A BLAST analysis was used to suggest cross reactivity with p65 (RelA) from human, mouse and rat based on 100% homology with the immunizing sequence. Cross reactivity with p65 (RelA) from other sources has not been determined.
Gene Name
RELA

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a c-terminal region near phospho Serine S536 of human p65 (RelA) protein.
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl

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