Polyclonal Chicken GFP Antibody (clone IQGFP6 Polyclonal, WB)
Catalog Number: LS-C827997-50
50 µL (1 mg/mL) / $492.00
Antibody: Chicken Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Format: Liquid
Anti-RBX1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC-plus™ Polyclonal Rabbit anti-Human RBX1 / ROC1 Antibody (aa94-108, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot |
| Reactivity: | Human |
| Format: | Liquid |
$526.00each
Catalog Number: LS-B415-50
Catalog Number: LS-B415
For bulk orders or custom formulations:
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- Description
- ROC1 antibody LS-B415 is an unconjugated rabbit polyclonal antibody to human ROC1 (RBX1) (aa94-108). Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
- Application
- ELISA, IHC, IHC-P, WB
- Reactivity
- Hu
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- RBX1 | Regulator of cullins 1 | RING box protein 1 | ROC1 | TESTICAN | Ring-box 1 | RNF75 | BA554C12.1 | Protein ZYP | RING finger protein 75 | RING-box protein 1 | ZYP protein
- Recommended Dilution: ELISA
- 1:2000 - 1:10000
- Recommended Dilution: IHC-P
- 1:500
- Recommended Dilution: WB
- 1:500 - 1:1000
- UniProt Number
- P62877
- NCBI GenBank
- NM_014248 | NP_055063.1
- SwissProt
- P62877
Target
- Target
- RBX1 / ROC1
- Antigen Species
- Human
- Specificity
- Reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.
- Epitope
- Reacts with human, mouse, C.elgans and zebra fish ROC1. Cross reactivity may also occur with ROC1 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC2 and APC11.
- Gene Name
- RBX1
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 94-108 of Human ROC1 (C-terminal) coupled to KLH.
- Immunogen Type
- Synthetic Peptide (Linear)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- Antiserum, 0.01% Sodium Azide
- Volume
- 50 Microliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Unpurified (Serum / Ascites / Supernatant)
Storage & Handling
- Storage Conditions
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase. Kamura T, Koepp DM, Conrad MN, Skowyra D, Moreland RJ, Iliopoulos O, Lane WS, Kaelin WG, Elledge SJ, Conaway RC, Harper JW, Conaway JW. Science (New York, N.Y.). 1999;284:657-61.
PubMed: 10213691
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Ohta T, Michel JJ, Schottelius AJ, Xiong Y. Molecular cell. 1999;3:535-41.
PubMed: 10230407
Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34. Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Deshaies RJ, Shevchenko A, Deshaies RJ. Genes & development. 1999;13:1614-26.
