LSBio
IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (clone rB22.1)

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (clone rB22.1)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Recombinant Monoclonal
Applications:Immunohistochemistry (general)
Reactivity:Human
Format:Liquid
See all KRT8 / CK8 / Cytokeratin 8 antibodies →
$470.00each
Catalog Number: LS-B16249-50

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Overview

Description
Cytokeratin 8 antibody LS-B16249 is an unconjugated mouse recombinant monoclonal antibody to human Cytokeratin 8 (KRT8 / CK8). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Application
IHC
Reactivity
Hu
Nonreactive Species
Chicken, Mouse, Rat, Xenopus
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Recombinant Antibodies
Alternative Names
CARD2 | CK-8 | CYK8 | Cytokeratin-8 | CK8 | K2C8 | K8 | KO | Keratin-8 | KRT8 | Type-II keratin Kb8 | Cytokeratin 8 | Keratin 8
Recommended Dilution: IHC-P
1:50
UniProt Number
P05787
NCBI GenBank
NM_002273 | NP_002264.1
SwissProt
P05787

Target

Target
KRT8 / CK8 / Cytokeratin 8
Antigen Species
Human
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.
Epitope
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.
Gene Name
KRT8

Antibody

Host Species
Mouse
Clonality
Recombinant Monoclonal
Clone
rB22.1
Isotype
IgG1
Origin Species
Human

Immunogen

Immunogen
Recombinant full-length human KRT8 protein. Antigen Molecular Weight: 52.5 kDa
Immunogen Type
Full-Length Recombinant Protein

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
10 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Concentration
200 µg/mL
Animal Free
No
Carrier-Free
No
Preservative
Yes
Purification Method
Protein A/G Affinity

Storage & Handling

Storage Conditions
Store at 2°C to 8°C for up to 2 years.
Recommended Storage Buffer
10 mM PBS

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.