Anti-KRT8 / Cytokeratin 8 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (clone M20)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Flow Cytometry, Immunocytochemistry, Immunohistochemistry (general), Immunohistochemistry, Frozen, Immunohistochemistry, Paraffin, Western Blot
Reactivity:	Human, Rabbit, Rat
Format:	Liquid

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$383.00each

Catalog Number: LS-B7166-0.05

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Overview

Description: Cytokeratin 8 antibody LS-B7166 is an unconjugated mouse monoclonal antibody to Cytokeratin 8 (KRT8 / CK8) from human. It is reactive with human, rat and rabbit. Validated for Flow, ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Application: FC, ICC, IHC, IHC-F, IHC-P, WB
Reactivity: Hu, Rb, Rt
Intended Use: Research Use Only
Guarantees: This product carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: CARD2 | CK-8 | CYK8 | Cytokeratin-8 | CK8 | K2C8 | K8 | KO | Keratin-8 | KRT8 | Type-II keratin Kb8 | Cytokeratin 8 | Keratin 8
Recommended Dilution: IHC-F: 1:100 - 1:200
Recommended Dilution: IHC-P: 10 µg/ml
Recommended Dilution: WB: 1:100 - 1:1000
UniProt Number: P05787
NCBI GenBank: NM_002273 | NP_002264.1
SwissProt: P05787

Target

Target: KRT8 / CK8 / Cytokeratin 8
Antigen Species: Human
Specificity: Reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiratory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of cytokeratin 8. Reacts with most mammals.
Epitope: Reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells and epithelia of the digestive, respiratory and urogenital tract. Also most adenocarcinomas derived from these cells are positive. The epitope has been mapped to amino acids 353-367 on the alpha helical rod domain of cytokeratin 8. Reacts with most mammals.
Gene Name: KRT8

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: M20
Origin Species: Human

Immunogen

Immunogen: Keratin isolated from the human breast carcinoma cell line MCF-7
Immunogen Type: Cell-Based Immunization

Format

Conjugate/Label: Unconjugated
Format: Liquid
Formulation: PBS, 0.09% Sodium Azide
Concentration: 1 mg/mL
Animal Free: No
Carrier-Free: Yes
Preservative: Yes
Purification Method: Other

Storage & Handling

Storage Conditions: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Recommended Storage Buffer: PBS