LSBio
IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (aa401-450)

Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

IHC-plus™ KRT8 / CK8 / Cytokeratin 8 Antibody (aa401-450)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human, Mouse, Rat
Format:Liquid
See all KRT8 / CK8 / Cytokeratin 8 antibodies →
$383.00each
Catalog Number: LS-B12422-100

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Overview

Description
Cytokeratin 8 antibody LS-B12422 is an unconjugated rabbit polyclonal antibody to Cytokeratin 8 (KRT8 / CK8) (aa401-450) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Application
ELISA, IF, IHC, IHC-P, WB
Reactivity
Hu, Ms, Rt
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
CARD2 | CK-8 | CYK8 | Cytokeratin-8 | CK8 | K2C8 | K8 | KO | Keratin-8 | KRT8 | Type-II keratin Kb8 | Cytokeratin 8 | Keratin 8
Recommended Dilution: IF/ICC
1:100 - 1:500
Recommended Dilution: IHC-P
1:50
Recommended Dilution: WB
1:500 - 1:1000
UniProt Number
P05787
SwissProt
P05787

Target

Target
KRT8 / CK8 / Cytokeratin 8
Antigen Species
Human
Specificity
Keratin 8 (Ab-431) Antibody detects endogenous levels of total Keratin 8 protein.
Epitope
Keratin 8 (Ab-431) Antibody detects endogenous levels of total Keratin 8 protein.
Gene Name
KRT8

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Synthetic peptide from human Keratin 8. (AA Range: 401-450)
Immunogen Type
Synthetic Peptide (Linear)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Volume
100 Microliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year.
Recommended Storage Buffer
PBS, 150 mM NaCl

Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. Casey AE, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, Cruickshank J, Shehata M, Drysdale E, Fang H, Kim H, Isserlin R, Bailey S, Medina T, Deblois G, Shiah YJ, Barsyte-Lovejoy D, Hofer S, Bader G, Lupien M, Arrowsmith C, Knapp S, De Carvalho D, Berman H, Boutros PC, Kislinger T, Khokha R. The Journal of cell biology. 2018 August;217:2951-2974.

PubMed: 29921600 · PMC: PMC6080920


PDGFRa+<sup> stromal adipocyte progenitors transition into epithelial cells during lobulo-alveologenesis in the murine mammary gland. Joshi PA, Waterhouse PD, Kasaian K, Fang H, Gulyaeva O, Sul HS, Boutros PC, Khokha R. Nature communications. 2019 April;10:1760.

PubMed: 30988300 · PMC: PMC6465250

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