HIF1A / HIF1 Alpha Antibody (clone HIF1A-84)
Catalog Number: LS-C357910
2 sizes / from $379.00
Antibody: Mouse Monoclonal
Applications: Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence
Reactivity: Human
Format: Liquid
Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa530-825)
Primary AntibodyLSBio Guarantee
| Antibody: | Rabbit Polyclonal |
| Applications: | Immunohistochemistry (general), Immunohistochemistry, Paraffin |
| Reactivity: | Goat, Hamster, Human, Mouse, Non-Human Primate, Rabbit, Rat |
| Format: | Liquid |
$383.00each
Catalog Number: LS-B495-0.1
Catalog Number: LS-B495
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- HIF1 Alpha antibody LS-B495 is an unconjugated rabbit polyclonal antibody to HIF1 Alpha (HIF1A) (aa530-825) from mouse. It is reactive with human, mouse, rat and other species. Validated for IHC. Cited in 7 publications.
- Application
- IHC, IHC-P
- Reactivity
- Gt, Ham, Hu, Ms, NHP, Rb, Rt
- Intended Use
- Research Use Only
- Guarantees
- This product carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | HIF1A | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
- Recommended Dilution: IHC-P
- 5 - 10 µg/ml
- UniProt Number
- Q16665
- NCBI GenBank
- NM_001530 | NP_001521.1
- SwissProt
- Q16665
Target
- Target
- HIF1A / HIF1 Alpha
- Antigen Species
- Mouse
- Specificity
- aa530-825 of human, mouse, rat, HIF-1 alpha
- Epitope
- HIF-1 alpha.
- Gene Name
- HIF1A
Antibody
- Host Species
- Rabbit
- Clonality
- Polyclonal
- Origin Species
- Human
Immunogen
- Immunogen
- A fusion protein including aa 530-825 of the mouse HIF-1 alpha protein.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Unconjugated
- Format
- Liquid
- Formulation
- PBS, 0.02% Sodium Azide
- Concentration
- 1 mg/mL
- Volume
- 0.1 Milliliter
- Animal Free
- No
- Carrier-Free
- Yes
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- PBS
Carbon ion radiotherapy of human lung cancer attenuates HIF-1 signaling and acts with considerably enhanced therapeutic efficiency. Subtil FS, Wilhelm J, Bill V, Westholt N, Rudolph S, Fischer J, Scheel S, Seay U, Fournier C, Taucher-Scholz G, Scholz M, Seeger W, Engenhart-Cabillic R, Rose F, Dahm-Daphi J, H[Character e4]nze J. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Mar;28:1412-21.
PubMed: 24347608
HIF-1 alpha signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Malec V, Gottschald OR, Li S, Rose F, Seeger W, H[Character e4]nze J. Free radical biology & medicine. 2010 Jun;48:1626-35.
PubMed: 20347035
Loss of HIF-1 alpha in the notochord results in cell death and complete disappearance of the nucleus pulposus. Merceron C, Mangiavini L, Robling A, Wilson TL, Giaccia AJ, Shapiro IM, Schipani E, Risbud MV. PloS one. 9:e110768.
PubMed: 25338007 · PMC: PMC4206488
ATF4 promotes bone angiogenesis by increasing VEGF expression and release in the bone environment. Zhu K, Jiao H, Li S, Cao H, Galson DL, Zhao Z, Zhao X, Lai Y, Fan J, Im HJ, Chen D, Xiao G. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2013;28:1870-1884.
PubMed: 23649506 · PMC: PMC4394202
Hypoxia-driven Hif2a coordinates mouse liver regeneration by coupling parenchymal growth to vascular expansion. Kron P, Linecker M, Limani P, Schlegel A, Kambakamba P, Lehn JM, Nicolau C, Graf R, Humar B, Clavien PA. Hepatology (Baltimore, Md.). 2016 December;64:2198-2209.
PubMed: 27628483
Hypoxia-induced autophagy drives colorectal cancer initiation and progression by activating the PRKC/PKC-EZR (ezrin) pathway. Komal Qureshi-Baig, Diana Kuhn, Elodie Viry, Vitaly I Pozdeev, Martine Schmitz, Fabien Rodriguez, Pit Ullmann, Eric Koncina, Martin Nurmik, Sonia Frasquilho, Petr V Nazarov, Nikolaus Zuegel, Marc Boulmont, Yervand Karapetyan, Laurent Antunes, Daniel Val, Michel Mittelbronn, Bassam Janji, Serge Haan, Elisabeth Letellier. Autophagy. 2019 Aug;16:1436-1452.
PubMed: 31775562 · PMC: PMC7469473
Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing. Aos S Karim, Aiping Liu, Christie Lin, Adam J Uselmann, Kevin W Eliceiri, Matthew E Brown, Angela L F Gibson. Wound repair and regeneration: official publication of the Wound Healing Society [and] the European Tissue Repair Society. 2020 November;28:812-822.
PubMed: 32686215 · PMC: PMC8592059
