LSBio
IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa432-528)

Anti-HIF1A antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

IHC-plus™ HIF1A / HIF1 Alpha Antibody (aa432-528)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity:Human
Format:Liquid
See all HIF1A / HIF1 Alpha antibodies →
$383.00each
Catalog Number: LS-B674-0.1

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Overview

Description
HIF1 Alpha antibody LS-B674 is an unconjugated rabbit polyclonal antibody to human HIF1 Alpha (HIF1A) (aa432-528). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Application
IHC, IHC-P, WB
Reactivity
Hu
Predicted Reactivity
Bovine, Mouse
Nonreactive Species
Rat
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
ARNT-interacting protein | ARNT interacting protein | BHLHe78 | HIF1 Alpha | HIF1A | Hypoxia-inducible factor1alpha | HIF-1A | HIF1 | MOP1 | Member of PAS superfamily 1 | PASD8 | HIF-1-alpha | HIF-1alpha | HIF1-ALPHA | Member of PAS protein 1
Recommended Dilution: IHC-P
10 µg/ml
Recommended Dilution: WB
1:1000
UniProt Number
Q16665
SwissProt
Q16665

Target

Target
HIF1A / HIF1 Alpha
Antigen Species
Human
Specificity
HIF-1 alpha.
Epitope
HIF-1 alpha.
Gene Name
HIF1A

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Origin Species
Human

Immunogen

Immunogen
Fusion protein containing amino acids 432-528 of human HIF-1alpha. Percent identity by BLAST analysis: Bovine, Porcine (93%); Mouse (90%).
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
PBS, 0.02% Sodium Azide
Concentration
1 mg/mL
Volume
0.1 Milliliter
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
PBS
Hypoxia pathways and cellular stress activate pancreatic stellate cells: development of an organotypic culture model of thick slices of normal human pancreas. Rebours V, Albuquerque M, Sauvanet A, Ruszniewski P, Lévy P, Paradis V, Bedossa P, Couvelard A. PloS one. 2013;8:e76229. PubMed: 24098783 PMC: PMC3786955

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