LSBio
IHC-plus™ Collagen VI Antibody

Anti-Collagen VI antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

IHC-plus™ Collagen VI Antibody

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin, Immunoprecipitation, Western Blot
Reactivity:Bovine, Human, Mammal (Other)
Format:Liquid
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$526.00each
Catalog Number: LS-B696-50

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Overview

Description
Collagen VI antibody LS-B696 is an unconjugated rabbit polyclonal antibody to Collagen VI from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Application
ELISA, IHC, IHC-P, IP, WB
Reactivity
Bov, Hu, Mml
Intended Use
Research Use Only
Guarantees
This product carries the LSBio 100% Guarantee. ✓
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Recommended Dilution: ELISA
1:5000 - 1:20000
Recommended Dilution: IHC-P
2.5 µg/ml
Recommended Dilution: IP
1:100
Recommended Dilution: WB
1:500 - 1:5000

Target

Target
Collagen VI
Antigen Species
Mammal (Other)
Specificity
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Epitope
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type VI collagens and has negligible cross-reactivity with Type I, II, III, IV or V collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Antibody

Host Species
Rabbit
Clonality
Polyclonal

Immunogen

Immunogen
Collagen Type VI from human and bovine placenta.

Format

Conjugate/Label
Unconjugated
Format
Liquid
Formulation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Animal Free
No
Carrier-Free
Yes
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Store vial at 4°C prior to opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below.
Recommended Storage Buffer
0.02 M Potassium Phosphate, 0.15 M NaCl
Accessibilome of human glioblastoma: collagen-VI-alpha-1 is a new target and a marker of poor outcome. Turtoi A, Blomme A, Bianchi E, Maris P, Vannozzi R, Naccarato AG, Delvenne P, De Pauw E, Bevilacqua G, Castronovo V. Journal of proteome research. Dec;13:5660-9. PubMed: 25325876
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